Abstract
A screening test was done to isolate microorganisms that produced nucleoside oxidase. The enzyme activity was found in a few strains of Gram-negative bacteria. One strain (LB-86) of these microorganisms showed strong ability to produce the enzyme. The morphological and physiological characteristics of strain LB-86 were studies, and this strain was found to be Pseudomonas maltophilia. From the examinations of culture conditions, suitable conditions for enzyme production were found, and strain LB-86 was aerobically cultured in shaking flasks at 26°C in a medium containing 2.5 % yeast extract, 3% glucose, 0.1% KH2PO4, 0.1% KCI, and 0.05% MgSO4•7H2O, pH 7.2. The highest activity was obtained after 22-24 hr of cultivation.
