Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Reduction of S-Sulfoglutathione by Cyanobacterial Ferredoxin-sulfite Reductase
Osamu KOGUCHIGoro TAMURA
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1989 Volume 53 Issue 3 Pages 783-788

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Abstract

S-Sulfoglutathione is reduced by reduced ferredoxin and ferredoxin dependent sulfite reductase (Fd-SiR) from the cyanobacterium Spimlina platensis. The Km value calculated from a Lineweaver-Burk plot of the data was 6.0 × 10-5M. A plot of ferredoxin concentration versus reduction activity of S-sulfoglutathione (GSSO3H) of Fd-SiR yielded a sigmoidal curve, giving a Hill coefficient (n) of 3.0. Antiserum against Fd-SiR strongly inhibits this reaction. In an experiment using a crude cell extract, the GSSO3H reducing activity was only found at the site of Fd-SiR in gel filtration and immunoblotting.

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