Abstract
The structure of αs1-casein at an emulsified oil/water interface was studied by immunochemical method using BALB/c mouse anti-αs1-casein antibody. By incubating the antiserum with an αs1-casein-stabilized emulsion, all of the specific antibodies in the serum were absorbed by the emulsion, suggesting that αs1-casein was adsorbed to the oil surface, all of its antigenic determinants being exposed to the aqueous phase. Since the antigenic determinants of αs1-casein for BALB/c mice are known to be present in the regions of residues 1 - 8, 33 - 54, 105 -119, 133 - 151, and 174 -199, these portions must be, at least partly, exposed to the aqueous phase and accessible to the antibodies.
