Isolation and Identification of Two Stimulators of PGI₂ Production from Fetal Calf Serum

Abstract

We isolated two major stimulators of PGI₂ production from fetal calf serum (PCS) by ultra filtration, Sephadex G-10 gel filtration and QAE-Sephadex column chromatography. These stimulators were identified as xanthine and uric acid.
The stimulatory effects on PGI₂ production of xanthine-related compounds and aromatic hydroxy compounds were examined using rat aortic rings. 2-Amino-6, 8-dihydroxypurine and 2, 8-dihydroxyadenine accelerated PGI₂ production, but guaiacol and 4, 4'-dihydroxycalcone greatly inhibited it.