Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Determination of Excreted 15N in Rats Fed on Nondialyzable Melanoidin Prepared from a Model System of Glucose and 15N-Glycine
Seiichi HOMMAYukiko ENDOMasao FUJIMAKI
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1989 Volume 53 Issue 7 Pages 1861-1865

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Abstract
Rats were fed for one day on a diet containing nondialyzable 15N-melanoidin prepared in a model system of glucose and 15N-glycine, and the excreta were collected over the following 7-day period. The total amounts of 15N excreted in the feces and in the urine were 80.9 % and 1.95%, respectively, of 15N in the melanoidin fed to the rats, indicating that melanoidin was difficult to excrete. The urea-15N content in the 15N of the urine was found to vary from 2.4 to 14.0 %, and the ratio of 15N in the urea to that in the urine was found to be large on the early and late days after the administration of 15N-melanoidin. Compared to the nondialyzable melanoidin fed to the rats, the melanoidin excreted in the feces was characterized by elevated percentages of melanoidin components with pi from 2.73 to 2.90 and of those retained at the charged area, and was difficult to be electroforesed by electrofocusirig.
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