Abstract
A continuous-flow sensor, in which sulfite oxidase (SOD) was immobilized on CNBr-activated Sepharose, was developed for the determination of sulfite in white wine. Hydrogen peroxide produced by the enzyme reaction was monitored with a platinum electrode, which was covered with a dialysis membrane. The response of the sensor was linear in the range of 1 - 10 ppm sulfite, with a correlation coefficent of 0.999. The relative standard deviation for 10 injections was 2.3 % at the 5 ppm sulfite level. The gelatin coagulation procedure was introduced for the removal of interfering substances in wine such as polyphenol compounds. The method was applied to automatic sample pretreatment.
