Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Purification and Properties of Allergenic Proteins in Buckwheat Seeds
Masayo YANORyo NAKAMURAShigeru HAYAKAWAShinpei TORII
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ジャーナル フリー

1989 年 53 巻 9 号 p. 2387-2392

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Three kinds of proteins (BA-1, BA-2 and BA-3) allergenic to the IgE antibody of allergenic individuals were isolated from buckwheat seeds. These three proteins were essentially homogeneous as judged by both polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The amino acid composition of BA-1 and BA-2 was very similar, and the molecular weight of each allergenic protein was between 8000-9000 by SDS-polyacrylamide gel electrophoresis. One of them was a trypsin inhibitor, and their immunoreactivity was quite stable to heating at 100°C for 60 min.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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