Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Production of Benzoylformic Acid from Phenylglycine by Saccharomycopsis lipolytica
Hiroshi KANZAKIAtsuhiko ISOBEYoshikazu IZUMIHideaki YAMADA
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1990 Volume 54 Issue 8 Pages 2101-2105


Microbial production of benzoylformic acid (BF), which can be used as a substrate of enzymatic synthesis of (R)-(-)-mandelic acid, was investigated. Among 145 strains of yeasts and actinomycetes, Saccharomycopsis lipolytica (IAM 4964) was the best producer of BF from DL-phenylglycine (DL-PG). Culture conditions for BF production by the organism were optimized. When 0.2% fructose as a carbon source and 0.7% Bacto-tryptone as a nitrogen source were used in the presence of 4% DL-PG, 14.5 mg/ml of BF was produced (about 37% molar yield) in 4 days of cultivation. BF was synthesized from the L-form of PG, but not from the D-form. The BF was isolated from culture broth in a crystalline form and physicochemically identified.

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