抄録
An α-amylase hyper producer A. oryzae and a protease hyper producer A. sojae were electrofused to breed more excellent koji-molds. Fusion between the yellow-spored, pant mutant or the yellow-spored, met mutant of A. oryzae and the white-spored, bio mutant or the white-spored, ade mutant of A. sojae produced heterokaryons with a frequency of 2 × 10-5 per protoplast pair. The optimal parameters for the fusion of Aspergillus protoplasts were 1.1 MHz AC frequency, 400 V/cm AC voltage, 4kV/cm DC pulse voltage, 60 μsec pulse duration, and 2 or 3 pulses. In this combination of the fusion-parents, all the fusants with stable green conidia formed an alkaline proteinase with the mobility of A. oryzae-type, and none of them formed any intermediate type. The productivity and molecular specificity of proteinases behaved independently from each other in the processes of fusion and haploidization.
