Transformation of Penidllium urticae with Plasmids Containing the Hygromycin B Resistance Gene

Abstract

A transformation system with efficiencies between 6 and 50 stable transformants per μg of DNA was developed for Penidllium urticae Jl (ATCC48163) using hygromycin B-resistant pi asm ids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 μg/ml hygromycin B, the transformants were resistant to more than 5 mg/ml 8of hygromycin B.