抄録
Rabbit polyclonal and mouse monoclonal antibodies raised against the previously isolated allergenic protein (RAP) detected several immunologically crossreactive proteins in the rice soluble protein fraction. Gel electrophoresis-immunoblotting analysis using the monoclonal antibody and isoelectric focusing-immunodiffusion analysis using the rabbit antibodies demonstrated that these proteins had molecular masses of about 14 to 16 kDa and isoelectric points of about 6 to 8. The rice albumin proteins had molecular masses of about 14 to 16 kDa and reacted well with the anti-RAP monoclonal antibody. These results suggested that the proteins responsible for rice allergy were major components with microheterogeneity in the rice albumin proteins.
