Isolation and Characterization of NAD(P)-Dependent L-Sorbosone Dehydrogenase from Gluconobacter melanogenus UV10
1991 年 55 巻 3 号 p. 665-670
詳細
1991 年 55 巻 3 号 p. 665-670
NAD(P)-Dependent L-sorbosone dehydrogenase has been purified from the cytosol fraction of Gluconobacter melanogenus UV10. The enzyme was purified about 30-fold with an overall yield of 19% by column chromatographies on DEAE-Sepharose CL-6B, DEAE-Sephadex A-50, and Blue Sepharose CL-6B. The enzyme required NAD or NADP as a cofactor, and showed broad substrate specificity on various aldehyde compounds. Among them, L-sorbosone was the best substrate for the enzyme. In this respect, the enzyme is a kind of aldehyde dehydrogenase.
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