Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Synthesis of Sialyllactose from N-Acetylneuraminic Acid and Lactose by a Neuraminidase from Arthrobacter ureafaciens
Isafumi MARUYasuhiro OHTAKaoru OKAMOTOShigeo SUZUKIKazuaki KAKEHIYoji TSUKADA
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1992 Volume 56 Issue 10 Pages 1557-1561

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Abstract

Two sialyllactose isomers, NeuAcα2→6Galβ1→4(NeuAcα2→6)Glc, were prepared by incubation of a concentrated solution of N-acetylneuraminic acid and lactose in the presence of a neuraminidase from Arthrobacter ureafaciens. Each sialyllactose was isolated by a combination of ion-exchange chromatography and high performance liquid chromatography. The structure of each sialyllactose was identified by mass spectrometry, nuclear magnetic resonance spectrometry, and enzymatic analysis.

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