Specific Measurement of a Major Mite Allergen, Der f II, by an Enzyme-linked Immunosorbent Assay System Using Monoclonal Anti-Der f II Antibodies

Abstract

An enzyme linked immunosorbent assay system using a monoclonal antibody, 15E11, specific for a major allergen Der f II in house dust mite, was developed. This system detected only Der f II in the presence of Der p II and other allergens. The Der f II contents in several house dust samples significantly correlated with the numbers of the mites in the same house dust samples (n=14, r=0.88, p<0.001). These data showed that this system was useful for specific measurement of Der f II in house dusts.