Alternative Secondary Structures in the Phage G4 Origin of the Complementary DNA Strand Synthesis : Effects of NaCl Concentration on the Bleomycin-DNA Interaction
Author's Organization：Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
The effects of NaCl concentration on bleomycin-induced cleavages of single-strand and double-strand DNA fragments containing the phage G4 origin of complementary DNA strand synthesis were investigated. It was found that bleomycin could be used as a reagent to analyze secondary and tertiary structures and subtle changes of DNA structures. The effects of NaCl concentration on cleavages of single-stranded DNA were distinct at every target site, indicating that the diversity of topolotical properties of DNA might change the selectivity of the bleomycin-induced DNA cleavage. These results showed alternative secondary structures within and close to the G4 origin of complementary DNA strand synthesis.
References (21)
Related articles (0)
Figures (0)
Content from these authors
Supplementary material (0)
Result List ()
Cited by
This article cannot obtain the latest cited-by information.
