Author's Organization：Hayashibara Biochemical Laboratories, Inc. Hayashibara Biochemical Laboratories, Inc. Hayashibara Biochemical Laboratories, Inc. Hayashibara Biochemical Laboratories, Inc. Hayashibara Biochemical Laboratories, Inc. Hayashibara Biochemical Laboratories, Inc. Hayashibara Biochemical Laboratories, Inc.
Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of oligosaccharides by the transglycosylation reaction with cyclomaltohexaose as a glycosyl donor and D-lactose as its acceptor. The content of oligosaccharide A as the main transfer product was 22. 1% of the total sugar. The content was increased to 34. 1% by hydrolysis with glucoamylase. Oligosaccharide A was isolated by column chromatography and crystallized. By chemical and enzymatic analyses, oligosaccharide A was found to be a novel non-reducing trisaccharide ; O-β-D-galactopyranosyl-(1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside.
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