Analysis of Phosphorylation of Wheat Elongation Factor 1β and β' by Casein Kinase II

Abstract

The purified casein kinase II (CK II) from Arabidopsis thaliana phosphorylates wheat elongation factor 1β (EF-1β), but not elongation factor 1β' (EF-1β'), which lacks a serine residue in the conserved phosphorylation site. Both EF-1β and β' subunits, with similar functions, seem to undergo different regulation despite the partial amino acid sequence of EF-1β being similar to that of EF-1β'.