Expression of an 87-kD-β-1, 3-Glucanase of Bacillus circulans IAM1165 in Saccharomyces cerevisiae by Low-temperature Incubation

Abstract

A DNA segment encoding a signal peptide from yeast invertase was fused in frame to bglH gene encoding 87-kD-β-1, 3-glucanase from Bacillus circulans IAM1165 and was expressed in the yeast Saccharomyces cerevisiae under the control of the GAL1 gene promoter. Yeast cells containing this fused gene produced active β-1, 3-glucanase in the medium after a long period of incubation at low temperature. The enzyme produced by yeast was heterogeneous in size, and larger than the enzyme produced by Escherichia coli.