Antitumor Protein-containing Polysaccharides from a Chinese Mushroom Fengweigu or Houbitake, Pleurotus sajor-caju (Fr.) Sings

Abstract

Protein-containing polysaccharides extracted from fruiting bodies of a Chinese fungus named Feng Wei Gu, were fractionated and purified, and their antitumor activities were tested, out of which the following active fractions were obtained. FI_o-a: A protein-containing xyloglucan, MW 280, 000, polysaccharide : protein=76 : 24 (w/w), polysaccharide consisting of Man : Gal : Xyl : Glc=2 : 12 : 42 : 42 (molar ratio). [α]²³_D +25.3°. FA-2: A protein-containing mannogalactan, MW 120, 000, polysaccharide : protein=76 : 16 (w/w), consisting of Xyl : Man : Gal=9 : 35 : 56 (molar ratio), [α]²³_D +98.5°. FII-1: A Protein-containing xylan (62 : 21 w/w). MW 200, 000, [α]²³_D +8.7°. FIII-1a: A protein-containing glucoxylan (15 : 71 w/w), [α]²³_D +30.7°, MW 90, 000, consisting of Glc : Xyl=40 : 44 (molar ratio). FIII-2a: A protein-containing xyloglucan, MW 70, 000, polysaccharide : protein=69 : 3 (w/w), polysaccharide consisting of Xyl : Glc=36 : 62 (molar ratio). [α]²³_D +38.6°.