Abstract
The rate parameters for maltooligosaccharides of α-glucosidase from Mucor javanicus were studied. The Km and ko values (mM, s-1) for maltose, malto-triose, -tetraose, -pentaose, -hexaose, and -heptaose were estimated to be (0.60, 334); (0.29, 271); (0.27, 312); (0.23, 285); (0.14, 230); (0.11, 184) in this order. The subsite affinities in the active site were 1.78, 5.31, 0.317, 0.126, 0.039, 0.168, and 0.002 kcal/mol for subsites 1, 2, 3, 4, 5, 6, and 7, respectively. From these results, it is considered that extended subsites exist in the enzyme, where maltooligosaccharides could be bound.
