Acetylcholinesterase Activity and Enzyme Kinetics in the Brain of Common Carp, Cyprinus carpio Subjected to Sub-lethal Exposure to Dimethoate

抄録

Dimethoate, an organophosphorus insecticide, inhibited the acetylcholinesterase of the brain of a common carp, Cyprinus carpio, by increasing the K_m without affecting the V_max. A Dixon's Plot confirmed the competitive nature of the inhibition, yielding a K_i of 2×10^-3M. The assay of brain acetylcholinesterase is thus useful in assessing pesticide toxicity to fishes.