Sequencing and Expression of Sheep Angiotensinogen cDNA

Masahiro Nagase; Fumiaki Suzuki; Akiyoshi Fukamizu; Natsuko Takeda; Kenichi Takeuchi; Kazuo Murakami; Yukio Nakamura

doi:10.1271/bbb.58.1884

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Sequencing and Expression of Sheep Angiotensinogen cDNA

Masahiro Nagase, Fumiaki Suzuki, Akiyoshi Fukamizu, Natsuko Takeda, Kenichi Takeuchi, Kazuo Murakami, Yukio Nakamura

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JOURNAL FREE ACCESS

1994 Volume 58 Issue 10 Pages 1884-1885

DOI https://doi.org/10.1271/bbb.58.1884

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Abstract

A sheep liver cDNA library was screened with a BstEII fragment of human angiotensinogen cDNA to obtain sheep angiotensinogen cDNA. The cDNA sequence showed that preangiotensinogen con-sisted of 452 amino acid residues (49 kDa) of angiotensinogen and 24 residues of a signal peptide. Recombinant sheep angiotensinogen (56kDa) was expressed in COS-7 cells.

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