Nuclear Magnetic Resonance Study and Secondary Structure Determination of the Antibiotic Peptide, Aibellin

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Aibellin is a 20-residue peptide antibiotic that has been isolated from the fungus Verticimonosporium ellipticum. Sequence-specific assignment of the ¹H- and ¹³C-NMR signals of aibellin in a methanol solution was achieved by using the two-dimensional NMR technique. Furthermore, its secondary structure was characterized by circular dichroism (CD) and NOESY spectra. The observed NOEs, ³J_NHCαH coupling constants and amide hydrogen-deuterium (H-D) exchange rates show that the peptide consisted of two α-helices and a bent structure around a Pro-14 residue.