A Novel Type of Proteinase, Masikinolysin, from Streptomyces griseoloalbus SN-22

Abstract

We found a novel type of proteinase, which we named masikinolysin, in the culture filtrate of Streptomyces griseoloalbus strain SN-22. The molecular weight and isoelectric point of the purified enzyme were determined to be 26, 000 and 6. 4, respectively. The optimum conditions were 45°C at pH 9. 0. From the cleavage sites of the insulin B-chain and lysozyme, the enzyme specifically hydrolyzed the carboxyl side of alanine and valine residues.