Screening for Milk-clotting Enzyme from Basidiomycetes

Hideyuki Kobayashi; Hyonok Kim; Takehiko Itoh; Kunihiro Kasamo; Isao Kusakabe; Kazuo Murakami

doi:10.1271/bbb.58.440

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Screening for Milk-clotting Enzyme from Basidiomycetes

Hideyuki Kobayashi, Hyonok Kim, Takehiko Itoh, Kunihiro Kasamo, Isao Kusakabe, Kazuo Murakami

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JOURNAL FREE ACCESS

1994 Volume 58 Issue 2 Pages 440-441

DOI https://doi.org/10.1271/bbb.58.440

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Abstract

Screening tests for aspartic proteinases with milk-clotting activity were done on basidiomycetes. Crude enzymes from 6 strains had a high ratio of milk-clotting activity to caseinolytic activity. These enzymes showed acidic pH optimum for proteolytic activity and were inhibited considerably by pepstatin, a specific aspartic proteinase inhibitor. Among them, the crude enzyme from Laetiporus sulphureus was more heat-labile than the other enzymes.

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