Abstract
Kojibiose and nigerose were found in a reaction mixture of sucrose phosphorylase from Leuconostoc mesenteroides using α-D-glucose-1-phosphate (G-1-P) as a donor and sucrose as an acceptor, and confirmed by 13C-NMR and HPLC analyses. The glucobioses were also detected using combinations of sugars such as sucrose and D-glucose, sucrose alone, or G-1-P and D-glucose, while none of them was detected using G-1-P alone or D-glucose alone. The efficiencies of the substrare on the formation of the glucobioses were as follows : sucrose and D-glucose > sucrose > G-1-P and D-glucose > G-1-P and sucrose.
