Fluorometric Assay of Glutathione Peroxidase Activity in Liver and Plasma with N- (9-Acridinyl)maleimide

Abstract

A sensitive assay of glutathione peroxidase activity by fluorometry with N-(9-acridinyl)maleimide was used to monitor the increase in GSSG with tert-butylhydroperoxide. The method was used to assay liver homogenates and human plasma samples, and was compared with a conventional coupled enzymatic method. The values, sensitivity, and precision of the new method were 1.0 to 1.6 times, 50 to 100 times, and 1.5 times, respectively, those by the conventional method.