Two Kinds of 2-Halo Acid Dehalogenases from Pseudomonas sp. YL Induced by 2-Chloroacrylate and 2-Chloropropionate

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The bacterial isolates WS, WL, and YL, which were identified as Pseudomonas, grew in 2-chloroacrylate (2-CAA) and 2-chloropropionate (2-CPA) media to produce 2-halo acid dehalogenases. Pseudomonas sp. YL showed the highest 2-CPA dehalogenase activity among the three strains, and inducibly produced two kinds of 2-halo acid dehalogenases in a medium containing either 2-CAA or 2-CPA. The 2-CAA-induced enzyme catalyzed dehalogenation of D- and L-2-CPA to produce L- and D-lactate, respectively. Its pH optimum of the dehalogenation and activity staining indicated that a single enzyme catalyzes the dehalogenation of both enantiomers of 2-CPA. The 2-CPA-induced enzyme catalyzed production of D-lactate from L-2-CPA, but did not act on D-2-CPA. The two dehalogenases are different from each other in mobility upon polyacrylamide gel electrophoresis and sensitivity towards thiol reagents.