Purification and Characterization of β-Glucosidase from Botrytis cinerea

Abstract

The β-glucosidase of a phytopathogenic fungus, B. cinerea, was purified and characterized. The optimum pH and temperature for activity of the enzyme were 3.0 and 60°C. The β-glucosidase was stable up to 50°C and between pH 4.0-10.0. The M_r was 380, 000. The affinity of the enzyme for oligosaccharides tended to increase in parallel with their chain length.