Substrate and Calcium Effects on the Autolysis of Tilapia Muscle m-Calpain

Abstract

The autolysis of tilapia m-calpain occurred even with very low free calcium at pH 5.5-7.5, and at 25°C or even 0°C. Substrates (casein mixture, α-casein, β-casein, fibrinogen, etc) affected the autolysis profile of calpain. Fragments from the degradation of α-casein seemed to inhibit the autolysis of the 80 kDa subunit.