Substrate Specificity of α-L-Arabinofuranosidase from Bacillus subtilis 3-6 toward Arabinofurano-oligosaccharides

Abstract

The substrate specificity of α-L-arabinofuranosidase (EC 3.2.1.55)from Bacillus subtilis 3-6 was explored using methyl 2-O-, methyl 3-O-, and methyl 15-O-α-L-arabinofuranosyl-α-L-arabinofuranosides, and methyl 3, 5-di-0-α-L-arabinofuranosyl-α-L-arabinofuranoside. The enzyme hydrolyzed the methyl α-L-arabinofuranobiosides to arabinose and methyl α-L-arabinofuranoside in the order of (1→2)- > (1→3)- > (1→5)-linkages. The enzyme hydrolyzed the (1→3)-linkage more than the (1→5)-linkage of the methyl α-L-arabinofuranotrioside.