Free and Conjugated Brassinosteroids in the Pollen and Anthers of Erythronium japonicum Decne.

Abstract

Both free and conjugated brassinosteroids (BRs) in the pollen and anthers of Erythronium japonicum Decne. were investigated. As a free form of BRs, typhasterol was identified by GC-MS. Polar conjugated BRs occurred only in the anther, and non-polar conjugated BRs occurred both in the pollen and mainly in the anther. BR parts of acid hydrolysis of the former were identified to be teasterone (major) and castasterone (minor). Those of alkaline hydrolysis of the latter were identitied to be typhasterol (major) and teasterone (minor).