Aromatization of 4-Oxocyclohexanecarboxylic Acid to 4-Hydroxybenzoic Acid by Two Distinctive Desaturases from Corynebacterium cyclohexanicum : Identification of the Intermediate

Abstract

The intermediate of the aromatization of 4-oxocyclohexanecarboxylic acid (OHA) to 4-hydroxybenzoic acid (HA) by Corynebacterium cyclohexanicum was identified as (+)-4-oxocyclohex-2-enecarboxylic acid (O2A) using a combined system of gas-liquid chromatography (GLC) and a mass spectrometer and polarimeter.