Enzymatic Syntheses of Two Stable (-)-Epigallocatechin Gallate-glucosides by Sucrose Phosphorylase

Abstract

Sucrose phosphorylase from Leuconostoc mesenteriodes catalyzed transglucosylation from sucrose to (-)-epigallocatechin gallate. Two main transfer products were obtained, and their structures were identified as (-)-epigallocatechin gallate 4'-O-α-D-glucopyranoside and (-)-epigallocatechin gallate 4', 4"-O-α-D-diglucopyranoside on the basis of secondary ion mass spectrometry analysis, component analyses of their enzymatic hydrolyzates, and nuclear magnetic resonance analysis. The stability of (-)-epigallocatechin gallate was greatly increased by the glucosylation.