Application of Micellar Electrokinetic Chromatography as a Novel Assay Method for Dimethyl Sulfoxide Reductase

Abstract

A direct and simple assay method for dimethyl sulfoxide reductase (DMSO reductase) by determining dimethyl sulfoxide (substrate) and dimethyl sulfide (product) in a reaction mixture of the enzyme was developed. Micellar electrokinetic chromatography (MEKC), a kind of capillary electrophoresis, was quite effective for a quantitative determination below the nanogram level without any pretreatment of the reaction mixture.