Purification and Characterization of an Aryl-alcohol Oxidase from the Lignin-degrading Basidiomycete Phanerochaete chrysosporium

Abstract

The activity of aryl-alcohol oxidase was detected in the mycelial extracts of a lignin-degrading basidiomycete, Phanerochaete chrysosporium. The induction of production of the enzyme by aryl-alcohols was suggested. The enzyme was purified to homogeneity. The molecular weight was estimated to be about 78, 000. The prosthetic group was found to be FAD. Several aryl alcohols can serve as substrates but aliphatic alcohols are inert.