Effects of MethylJasmonate and Concanavalin A on Embryogenesis and the Induction of Secondary Somatic Embryos of Carrot

抄録

When embryogenic cell clusters prepared in a novel embryo-forming system were cultured in a standard liquid medium that contained methyljasmonate (MeJ), their development invariably proceeded to the torpedo stage but, thereafter, their morphological development to plantlets was suppressed to a considerable extent. Plantlets that occasionally regenerated in the presence of MeJ were very dwarfed. Secondary somatic embryogenesis was observed to occur at a frequency on the surface of embryos that remained at the torpedo stage and on dwarfed plantlets. Similar results were obtained with concanavalin A (Con A). These secondary embryos became developed normally into plantlets after transfer to a liquid medium without MeJ and Con A.