Measurement of Positional Specificity Index of Microbial Lipases by Chiral Phase High-pressure Liquid Chromatography

Yasuhide OTA; Yutaka ITABASHI; Masaki HASUO

doi:10.1271/bbb.60.145

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Measurement of Positional Specificity Index of Microbial Lipases by Chiral Phase High-pressure Liquid Chromatography

Yasuhide OTA, Yutaka ITABASHI, Masaki HASUO

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Keywords: lipase, positional specificity, stereoselectivity

JOURNAL FREE ACCESS

1996 Volume 60 Issue 1 Pages 145-146

DOI https://doi.org/10.1271/bbb.60.145

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Abstract

The positional specificity index (PSI) of microbial lipases was measured using chiral phase high-pressure liquid chromatography (HPLC). The PSI value calculated was more precise than that using thin-layer chromatography, especially when the PSI was near 100.

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