Author's Organization：Department of Molecular Biology, National Institute of Bioscience and Human-Technology Instituto Nacional De Tecnologioa Y Normalizacion Ingeniero Quimico, Av. Artigas Y, Denis Roa, Asuncion, Paraguay Chemistry Department of Malaysia National Research Institute of Brewing Department of Molecular Biology, National Institute of Bioscience and Human-Technology
We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridizationi using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII; fragment contained the entire structural gene enoA including 5'-and 3'-flanking regions. The homology between A. oryzae enoA and S.cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A.oryzae.
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