Simple Affinity Purification Method for Raw Starch-adsorbable and -digesting Amylases with a Raw Starch Column

Abstract

A simple purification procedure for raw starch-adsorbable and -digesting amylases (RSAs) was devised. The method depended on an affinity column, which was prepared by mixing raw corn starch and Hyflo Super-Cel. RSAs were specifically adsorbed on the matrix, and eluted with a buffer containing 1% β-cyclodextrin. This column could be used to purify RSAs from Streptomyces thermo-cyaneoviolaceus and a recombinant strain of E. coli.