Effect of Reaction Temperature on the Determination of Rat Serum IgE by the Avidin-Biotin Method

Abstract

We established an avidin-biotin method for the sensitive determination of rat IgE and found that a non-specific signal was generated depending on the reaction temperature. When the sera of rats immunized or not with ovalbumin (OVA) were fractionated in a hydroxyapatite column and OVA-specific IgE was determined by the avidin-biotin method at 4°C or 37°C, OVA-specific IgE peaks were detected at 37°C, even with nonimmunized rats, but not at 4°C.