Cloning and Characterization of One Member of the Chalcone Synthase Gene Family from Solanum tuberosum L.

Jae Heung JEON; Hyun Soon KIM; Kyung Hwa CHOI; Young Hee JOUNG; Hyouk JOUNG; Si Myung BYUN

doi:10.1271/bbb.60.1907

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Cloning and Characterization of One Member of the Chalcone Synthase Gene Family from Solanum tuberosum L.

Jae Heung JEON, Hyun Soon KIM, Kyung Hwa CHOI, Young Hee JOUNG, Hyouk JOUNG, Si Myung BYUN

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Keywords: chalcone synthase, cDNA, Solanum tuberosum

JOURNAL FREE ACCESS

1996 Volume 60 Issue 11 Pages 1907-1910

DOI https://doi.org/10.1271/bbb.60.1907

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Abstract

We have isolated a chalcone synthase gene 2 (ST-CHS2) from potato by rapid amplification of cDNA ends by PCR. CHS2 cDNA had high homology to tomato LET-CHS2 (98%), petunia PHCHSJ (94%), potato ST-CHS1B (92%), petunia PHCHSA (92%), and LET-CHS1 (90%) at the overall 389-amino acid level. Genomic hybridization analysis indicated that CHS genes of potato comprise a family of at least six individual members.

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