The Fate of Mn²⁺ Ions Inside Saccharomyces cerevisiae Cells Seen by Electron Paramagnetic Resonance

Abstract

The fate of Mn²⁺ inside Saccharomyces cerevisiae cells was monitored during import and export processes, using information from electron paramagnetic resonance (EPR) spectroscopy analysis. EPR spectra showed that when entering the cell in high number, manganese ions immediately precipitated. If recorded under conditions that favored manganese efflux, EPR spectra indicated that only osmotically free ions were exported and that bound manganese had to turn into the soluble form before being able to leave the cell.