Two Forms of α-Glucosidase from Mucor javanicus Produced by Culture with Castanospermine

Abstract

When Mucor javanicus was cultured with castanospermine, two α-glucosidases (CS-I, II) were produced. The two enzymes were purified by two CM-cellulofine column chromatographies, and compared with the α-glucosidase (C) from a culture without castanospermine. The molecular weights of the three enzymes were calculated to be 97, 000 (CS-I, II) and 94, 000 (C) from SDS-PAGE. CS-II was similar in hydrolysis of α-1, 4-linkage to C, but hydrolyzed α-1, 2-, α-1, 3-, and α-1, 6-linkages at a lower rate than C. The Michaelis constant of CS-I for malto-oligosaccharides was several times as high as the constants of CS-II and C, but CS-I hydrolyzed nigerose at a faster rate than maltose.