Application of Long-distance PCR to Restriction Site Mapping of a Cloned DNA Fragment on the λEMBL3 Phage Vector
1996 年 60 巻 6 号 p. 1011-1013
詳細
1996 年 60 巻 6 号 p. 1011-1013
Long-distance PCR was applied to rapidly map the restriction sites of long inserts cloned on λEMBL3 phage vector. The restriction sites of 9 of 15 enzymes were completely assigned in a model experiment within 14 h, including 8 h for the PCR amplification. This method was found particularly useful for genomic DNA cloning when the partial sequence of the corresponding cDNA is known.
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