1996 Volume 60 Issue 6 Pages 975-978
Starch degradation was not increased under dark-anaerobiosis compared with that under dark-aerobic or light-anaerobic conditions in a green alga, Chlamydomonas sp. MGA161, though was increased in a control, C. reinhardtii. Orthophosphate (Pi) stimulated starch degradation and increased ethanol and glycerol excretion while decreasing H2 and acetic acid formation in strain MGA161. Phosphofructokinase was little increased in cells treated with Pi. Incubation with Pi inhibited hydrogenase activity in intact cells, and was accompanied by stimulation of starch degradation. Addition of H2 to the fermentative gas phase resulted in uptake of the H2 and the disappearance of H2 evolution, which in turn raised the rate of starch degradation while increasing the excretion of ethanol and glycerol and decreasing the release of acetic acid. It is suggested that the starch degradation is increased not in a state that the ATP neogenesis through proton motive force does not function in dark-anaerobiosis but in a state that the reducing equivalence is increased by H2 uptake and repression of the release.
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