Acceptor Specificity of Cyclodextrin Glucanotransferase from an Alkalophilic Bacillus Species and Synthesis of Glucosyl Rhamnose

Abstract

Cyclodextrin glucanotransferase [1, 4-α-D-glucan 4-α-D-(1, 4-α-D-glucano)-transferase (cyclizing), EC 2.4.1.19] from an alkalophilic Bacillus species A2-5a had a wider acceptor specificity than that from B. macerans, which was similar to those from B. stearothermophilus and B. circulans. Glucosyl rhamnose produced by the CGTase was identified as glucopyranosyl-α-1, 4-rhamnopyranose by α- and β-glucosidase treatments, and ¹H- and ¹³C-NMR analyses.