Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
Tsutomu KAKIMORITadashi YOSHlMOTOHiroshi OYAMANaoko ODAYoshikazu GOTOHKohei ODASawao MURAODaisuke TSURU
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JOURNAL FREE ACCESS

1996 Volume 60 Issue 7 Pages 1210-1211

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Abstract

A chromosomal DNA fragment of Scytalidium lignicolum that encodes the mature enzyme region of acid protease B (Scytalidopepsin B), was cloned and its nucleotides sequenced. The fragment contained a 76-bp intron at the middle of the mature enzyme-coding region. The mature enzyme was composed of 206 amino acid residues with a molecular weight of 21, 550. There were some discrepancies between the amino acid sequence deduced from these results and that previously established by protein sequencing.

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