Expression of 135-kDa Insecticidal Protein Gene from Bacillus thuringiensis in the Yeast Saccharomyces cerevisiae

Kayo WATANABE; Masatake TOYODA; Hideaki HASHIMOTO; Kei-ichi NAKAGAWA; Keiko NAKAMURA; Yoshiyasu YABUSAKI; Akio TANIMURA; Yukio SAITO

doi:10.1271/bbb.60.1483

Kayo WATANABE, Masatake TOYODA, Hideaki HASHIMOTO, Kei-ichi NAKAGAWA, Keiko NAKAMURA, Yoshiyasu YABUSAKI, Akio TANIMURA, Yukio SAITO

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Keywords: Bacillus thuringiensis, insecticidal protein, cryIA (a), Saccharomyces cerevisiae, Plutella xylostella larvae

JOURNAL FREE ACCESS

1996 Volume 60 Issue 9 Pages 1483-1485

DOI https://doi.org/10.1271/bbb.60.1483

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Abstract

Bacillus thuringiensis subsp. aizawai produces 130-kDa and 135-kDa (CryIA(a)) insecticidal proteins. When Saccharomyces cerevisiae was transformed by the vector carrying a cryIA(a) gene, the gene expression could not be observed. When the 5'-upstream region from the initiation codon was removed using a synthetic oligonucleotide, the CryIA(a) protein was successfully synthesized in yeast. The yeast extract containing CryIA(d) protein had insecticidal activity against Plutella xylostella larvae.

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