Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase

Tomonori NAKAI; Naoto TONOUCHI; Takayasu TSUCHIDA; Hitoshi MORI; Fukumi SAKAI; Takahisa HAYASHI

doi:10.1271/bbb.61.1955

Tomonori NAKAI, Naoto TONOUCHI, Takayasu TSUCHIDA, Hitoshi MORI, Fukumi SAKAI, Takahisa HAYASHI

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Keywords: recombinant enzyme, sucrose, asymmetrically labeling, mung bean

JOURNAL FREE ACCESS

1997 Volume 61 Issue 11 Pages 1955-1956

DOI https://doi.org/10.1271/bbb.61.1955

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Abstract

About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[¹⁴C]glucose or [¹⁴C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.

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